Nanometric molecular separation measurements by single molecule photobleaching.

摘要: Although considerable progress has been made in imaging distances cells below the diffraction limit using FRET and super-resolution microscopy, methods for determining separation of macromolecules 10-50 nm range have elusive. We developed fluorophore localisation with photobleaching (FLImP), based on quantised bleaching individual protein-bound dye molecules, to quantitate molecular separations oligomers nanoscale clusters. demonstrate benefits our method studying nanometric organisation epidermal growth factor receptor cells.