Macrophages are a source of extracellular adenosine deaminase-2 during inflammatory responses.

摘要: Serum activity of the adenosine deaminase (ADA) isozyme, ADA2, has been reported to be elevated during various disease states. Macrophages have suggested as cellular source extracellular ADA because they are one only cell types in which intracellular ADA2 measured, but secretion never demonstrated. Rat primary peritoneal macrophages (PPMs) and peripheral blood monocytes (PBMs) were harvested incubated for 18 h RPMI supplemented with horse serum. PPM PBM lysates assayed (ammonia production). In vitro vivo activities measured media rat serum, respectively. Activity ADA1 was confirmed by selective inhibition erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA). inhibited 2'-deoxycoformcin only, increased at a low pH (6.5). both isozymes found PPMs PBMs, their media. separate group rats, peritonitis induced ip insertion 400 mg/kg caecal slurry. 24 later h. from rats similar proportion. contrast, these had lower higher compared nonseptic rats. This resulted greater proportion The isozyme proportions serum septic more closely resembled that response small relative PPM. These results suggest significant isozymes, increases an inflammatory response. Because profiles differ concentrations, data also differential release each PPMs.