Recommandations techniques et règles de bonne pratique pour la coloration de May-Grünwald-Giemsa : revue de la littérature et apport de l’assurance qualité

摘要: May-Grunwald-Giemsa (MGG) stain is a Romanowsky-type, polychromatic as those of Giemsa, Leishman and Wright. Apart being the reference method haematology, it has become routine diagnostic cytopathology for study air-dried preparations (lymph node imprints, centrifuged body fluids fine needle aspirations). In context their actions promoting principles quality assurance in cytopathology, French Association Quality Assurance Anatomic Cytologic Pathology (AFAQAP) Society Clinical Cytology (SFCC) conducted proficiency test on MGG 2013. Results from test, together with review literature data allow pre-analytical analytical steps to be updated. Recommendations include rapid air-drying cell preparations/imprints, fixation using either methanol or May-Grunwald alone 3-10minutes, two-step staining: 50% buffer pH 6.8 v/v 3-5minutes, followed by 10% buffered Giemsa solution 10-30minutes, running water 1-3minutes. evaluation must performed red blood cells (RBCs) leukocytes, not tumour cells. Under correct conditions, RBCs appear pink-orange (acidophilic) buff-coloured, neither green nor blue. Leukocyte cytoplasm almost transparent, clearly delineated granules. However, staining may vary somewhat testing recommended automated methods (slide stainers) which remain standard reproducibility. Though remains stain, Diff-Quik(®) can used samples.