Affinity capillary electrophoresis of DNA for detection of single-nucleotide polymorphisms and point mutations Comprehensive study for optimization of the weak affinity
作者： Kae Sato , Miwa Onoguchi , Kazuo Hosokawa , Mizuo Maeda
DOI: 10.1016/J.CHROMA.2005.10.020
关键词:
摘要: Abstract A single-stranded DNA and its point mutant can be separated with affinity capillary electrophoresis (ACE) in which an appropriate ligand is used as a pseudo-stationary phase. In this paper, we systematically examine the effects of sequence, temperature, cation concentration on ACE separation quality, quantitatively evaluated terms resolution peak height. For fine tuning affinity, control MgCl2 insertion spacer sequence into are more effective than temperature. design DNA, simple strategy proposed, based prediction melting This was tested eleven different sample sequences. All them were under same conditions (250 μM 25 °C), nine cases had satisfactory qualities.
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