Rabbit Model of Candida Keratomycosis

摘要: Publisher Summary This chapter presents a rabbit model of Candida keratomycosis in which male rabbits 3.5–4.5 kg body weight were used. To exclude bacterial superinfection, gentamicin sulphate 0.5% eye drops or chloramphenicol 0.2% drops, both without preservatives, instilled twice daily. The efficacy this measure was verified by weekly examinations including an antibiogram (DIN 59840). animals anaesthetized with 5 mg/kg each Rompun and Ketavet. fungal suspension injected directly into the cornea through 27G cannula. In 17 eyes, infective strain used albicans DSM 70010, vitro displayed strong germ-tube formation after 24-hour incubation at 37° C glucose bouillon aqueous humor. Parallel experiments vivo performed on six eyes using CBS 2730, under same conditions, did not show formation. preliminary experiments, individual components yeast intracorneally 2 to differentiate between their effects that full suspension. check progress infection, findings examined slit-lamp microscopy documented photographically daily during first week thereafter every other day from second until end experiment. evaluation criteria adopted size infiltrates ulcers, presence hypopyon, extent reactive corneal vascularization, occurrence descemetocele/corneal perforation. After observation period, killed overdose enucleated.