Human vascular endothelial cells stimulate memory but not naive CD8+ T cells to differentiate into CTL retaining an early activation phenotype.

摘要: Endothelial cell (EC)-selective alloreactive CTL may mediate alloimmune vascular injury. In the present study, EC-selective were generated in cocultures of purified human CD8+ T cells with allogeneic EC and compared conventional against corresponding B lymphoblastoid (BLC). caused activation expansion memory but not naive cells, which differentiated into that retained high surface expression CD69, CD25, CD62L displayed low intracellular perforin content. contrast, BLC-stimulated could be from or showed a more mature phenotype (low higher levels perforin). The by stimulation was 5- to 20-fold less effective than cultures, accounting for reduction assayable cytotoxicity individual microcultures. these IL-2-supplemented cocultures, no effect on generation observed mAb blocking costimulation provided LFA-3, ICAM-1, CD40, addition comitogenic anti-CD28 mAb, preactivation CD40 ligand. Cyclosporine inhibited similarly both EC- cultures did affect those emerge. This study extends characterization endothelium as an immunoregulatory type distinct APC explain why graft rejection within arterial intima, anatomic compartment primary APC, is separable parenchyma.