Functionalization of whole-cell bacterial reporters with magnetic nanoparticles

摘要: We developed a biocompatible and highly efficient approach for functionalization of bacterial cell wall with magnetic nanoparticles (MNPs). Three Acinetobacter baylyi ADP1 chromosomally based bioreporters, which were genetically engineered to express bioluminescence in response salicylate, toluene/ xylene alkanes, functionalized 18 3 nm iron oxide MNPs acquire function. The efficiency bioreporters was 99.96 0.01%. MNPs-functionalized (MFBs) can be remotely controlled collected by an external field. MFBs all viable functional as good the native cells terms sensitivity, specificity quantitative response. More importantly, we demonstrated that salicylate sensing applied sediments garden soils, semiquantitatively detect those samples discriminably recovering permanent magnet. magnetically are especially useful complex environments indigenous cells, particles impurities may interfere direct measurement bioreporter conventional filtration is not applicable distinguish harvest bioreporters. described here provides powerful tool control selectively manipulate MNPs-unctionalized water soils. It would have potential application environmental microbiology, such bioremediation enhancement environment monitoring assessment.