Capsids Are Formed in a Mutant Virus Blocked at the Maturation Site of the UL26 and UL26.5 Open Reading Frames of Herpes Simplex Virus Type 1 but Are Not Formed in a Null Mutant of UL38 (VP19C)

摘要: Previously we reported that null mutant viruses of UL19 (VP5) or UL18 (VP23), essential components herpes simplex virus type 1 (HSV-1) capsid shells, do not form precursor structures as judged by sedimentation and electron microscope analysis. A goal the present experiments was to isolate a for remaining component VP19C, encoded UL38 open reading frame (ORF). Furthermore, wished determine if altered in UL26 maturation cleavage site at residues 610 611 produced lethal phenotype. Therefore, decided cell lines encode express multiple genes. Several were isolated transformation Vero cells one designated C32 expressed all proteins. Using this line ORF UL26.5 gene products. We found VP19C did detectable product analyses following infection nonpermissive cells. The resulted accumulation B capsids. capsids into C Interestingly, absence required retention VP24 capsid.