Generation of PM2 DNA breaks in the course of reduction of chromium(VI) by glutathione.

摘要: The carcinogen chromate is efficiently taken up and reduced to chromium(III) compounds by various biological systems. To test the possible DNA damage induced in course of chromium(VI) reduction, we used a combination with reductant glutathione (GSH) as well green complex chromium(V), which formed reaction GSH. was found cause single-strand breaks supercoiled circular bacteriophage PM2. chromium(V) Na4(GSH)4Cr(V).8H2O, prepared from glutathione, also cleaved PM2 DNA. No DNA-degrading effects were observed either or final product GSH, purple anionic GSH complex. nature buffering agents revealed strong influence on extent strand produced A variation concentration DNA, performed sodium phosphate-buffered solutions showed an initial increase number at concentrations 1 mM followed decline higher concentrations. Since neither chromate, when administered individually, nor complex, any detectable cleavage, critical steps leading occur conversion most abundant intracellular low molecular thiol. Moreover, demonstration that cleavage presence identifies oxidation state metal, involved DNA-damaging chromate.