Transcriptome analysis of G protein-coupled receptors in distinct genetic subgroups of acute myeloid leukemia: identification of potential disease-specific targets

摘要: Acute myeloid leukemia (AML) is associated with poor clinical outcome and the development of more effective therapies urgently needed. G protein-coupled receptors (GPCRs) represent attractive therapeutic targets, accounting for approximately 30% all targets marketed drugs. Using next-generation sequencing, we studied expression 772 GPCRs in 148 genetically diverse AML specimens, normal blood bone marrow cell populations as well cord blood-derived CD34-positive cells. Among these receptors, 30 are overexpressed 19 downregulated samples compared Upregulated enriched chemokine (CCR1, CXCR4, CCR2, CX3CR1, CCR7 CCRL2), adhesion (CD97, EMR1, EMR2 GPR114) purine (including P2RY2 P2RY13) receptor subfamilies. The include GPCRs, such LPHN1, GPR125, GPR56, CELSR3 GPR126, protease-activated (F2R F2RL1) Frizzled family SMO FZD6. Interestingly, specific deregulation was observed distinct subgroups AML, thereby identifying different potential frequent subgroups.