Chronic shisha exposure alters phosphoproteome of oral keratinocytes.
作者： Shankargouda Patil , Pavithra Rajagopalan , Krishna Patel , Tejaswini Subbannayya , Niraj Babu
DOI: 10.1007/S12079-019-00528-4
关键词:
摘要: Shisha smoking has been epidemiologically linked to oral cancer. However, few studies have investigated the pathobiology of shisha-induced cellular transformation. We studied effects chronic shisha exposure (8 months) in an vitro model using immortalized, non-neoplastic keratinocytes (OKF6/TERT1). Quantitative proteomic and phosphoproteomic analyses were performed on OKF6/TERT1 cells treated with extract for a period 8 months. Pathway analysis was carried out identify significantly enriched biological processes shisha-treated cells. Chronic resulted increased cell scattering phenomenon Data revealed differential phosphorylation 164 peptides (fold change ≥1.5, p ≤ 0.0.5) corresponding 136 proteins. Proteins associated mTORC1 EIF4F complexes involved initiating protein translation seen be upon treatment. Network also highlighted downregulation proteins Type I interferon signaling approach elucidated global perturbations molecular milieu exposure. Further are needed validate putative targets cancer patients history.
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