Secretion of melanin-concentrating hormone and neuropeptide-EI from cultured rat hypothalamic cells.

摘要: Rat melanin-concentrating hormone (MCH) is a homolog of the peptide first isolated from salmon pituitary glands which produces melanosome aggregation within melanophores teleost fish as well interacting with hypothalamic-pituitary axis. We have previously characterized rat and human MCH counterparts identical 19-amino acid peptides showing approximately 70% sequence identity to MCH. Immunoreactivity for has been found in high concentrations cell bodies dorsolateral hypothalamus, projections hippocampus, brainstem, posterior pituitary, cerebral cortex. adapted cultured model studying secretory responses hypothalamic cells obtained 7-day-old rats. precursor-derived neuropeptide glutamic isoleucine (NEI) were secreted these after 2 days culture up 22-24 culture. The confirmed by HPLC analysis RIA be isolat...