mRNA vaccination with charge-altering releasable transporters elicits human T cell responses and cures established tumors in mice.
作者： Ole A. W. Haabeth , Timothy R. Blake , Colin J. McKinlay , Robert M. Waymouth , Paul A. Wender
关键词:
摘要: In vivo delivery of antigen-encoding mRNA is a promising approach to personalized cancer treatment. The therapeutic efficacy vaccines contingent on safe and efficient gene delivery, biological stability the mRNA, immunological properties vaccine. Here we describe development evaluation versatile highly vaccine-delivery system that employs charge-altering releasable transporters (CARTs) deliver antigen-coding antigen-presenting cells (APCs). We demonstrate in human peripheral blood mononuclear CART can activate robust antigen-specific immune response against mRNA-encoded viral epitopes. an established mouse model, CARTs preferentially target professional APCs secondary lymphoid organs upon i.v. injections local s.c. injection. Finally, show coformulated with Toll-like receptor ligand simultaneously transfect generate treat cure mice large, tumors.
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