Studies of protein—Protein interaction using countercurrent distribution in aqueous two-phase systems: Partition behavior of five glycolytic enzymes from crude baker's yeast extract

摘要: Abstract The partition behavior of five glycolytic enzymes, in extracts from baker's yeast (Saccharomyces cerevisiae), between two aqueous phases has been studied by countercurrent distribution. All enzymes showed distribution patterns which indicated homogeneity and a similar behavior. In purified form, three the (glyceraldehyde-phosphate dehydrogenase, 3-phosphoglycerate kinase, enolase) same as extracts. Pure 6-phosphofructokinase, on other hand, changed its distinctively relative to what was found These results indicate interactions this enzyme macromolecular compounds support model suggested Kurganov et al. (1985, J. Theor. Biol. 116, 509–526 ) describing “glycolytic particle.”