Integrated RNA- and protein profiling of fermentation and respiration in diploid budding yeast provides insight into nutrient control of cell growth and development.

摘要: Abstract Diploid budding yeast undergoes rapid mitosis when it ferments glucose, and in the presence of a non-fermentable carbon source absence nitrogen triggers sporulation. Rich medium with acetate is commonly used pre-sporulation medium, but our understanding molecular events underlying acetate-driven transition from to meiosis still incomplete. We identified 263 proteins for which mRNA protein synthesis are linked or uncoupled fermenting respiring cells. Using motif predictions, interaction data RNA profiling we find among them 28 likely targets Ume6, subunit conserved Rpd3/Sin3 histone deacetylase-complex regulating genes involved metabolism, stress response meiosis. Finally, identify 14 both detected exclusively cells not sample set, including CSM4 , SPR1 SPS4 RIM4 were thought be meiosis-specific. Our work reveals intertwined transcriptional post-transcriptional control mechanisms acting MAT /α strain responds nutritional signals, provides clues how primes entering Biological significance integrated genomics study insight into interplay between transcriptome proteome diploid undergoing vegetative growth glucose (fermentation) (respiration). Furthermore, novel target these processes DNA binding deacetylase Rpd3 co-repressor Sin3. have combined an experiment using tiling arrays that cover entire genome, large-scale detection analysis based on mass spectrometry This distinguishes most others field—which investigate haploid strains—because only can undergo meiotic development simultaneous nitrogen. Indeed, report respiration might prime efficient spore formation, phenomenon well known poorly understood.