An on-line coupling of nanofibrous extraction with column-switching high performance liquid chromatography - A case study on the determination of bisphenol A in environmental water samples.

摘要: Abstract Polyamide 6 nanofiber polymers were used as modern sorbents for on-line solid phase extraction (SPE) coupled with liquid chromatography. The SPE system was tested the determination of bisphenol A in river water samples. nanofibers prepared using needleless electrospinning, inserted into a mini-column cartridge (5 × 4.6 mm) and HPLC. effect column packing amount polyamide on efficiency process optimized. proposed method performed 50-µL sample injection followed by an nanofibrous procedure. influence washing mobile retention during procedure evaluated. Ascentis® Express C18 (10 cm core-shell analytical column. Fluorescence detection wavelengths (λex = 225 nm λem 320 nm) identification quantification Bisphenol waters. linearity range from 2 to 500 µg L−1 (using nine calibration points). limits 0.6 2 µg L−1, respectively. developed successfully various samples waters Czech Republic (The Ohře, Labe, Nisa, Upa, Opava Rivers).