Cloning and analysis of the promoter region of the rat SM22α gene

摘要: We have cloned and sequenced a 1.9 kb fragment of the 5'-upstream sequence smooth-muscle-specific gene SM22 alpha. The region consisted alpha promoter, 65 bp exon containing most 5'-untranslated 307 first intron. A 1.5 at 5' end this was able to drive expression reporter chloramphenicol acetyltransferase (CAT) in both vascular smooth-muscle cells Rat-1 fibroblasts. This promoter did not contain consensus TATAA box but contained TTTAAA 25 from major start site identified by primer extension. Deletion analysis showed that +65 -303 more active cell types than suggesting there are silencer sequences core promoter. CAT activity also observed with fragments bases -193 -117 cells. In contrast cells, no detected fibroblasts smallest two fragments. residual tested suggested that, unlike alpha-actin transcription can occur absence factors binding CC(A/Trich)6GG (CArG box) or CANNTG (E motifs.