The calcification potential of human MSCs can be enhanced by interleukin-1β in osteogenic medium.
作者： Claudia Loebel , Ewa M Czekanska , Judith Staudacher , Gian Salzmann , R Geoff Richards
DOI: 10.1002/TERM.1950
关键词:
摘要: Inflammation is one of the key regulators repair process in bone tissues. Current data about effect interleukin-1β (IL-1β) on MSCs and osteoblasts are conflicting. We investigated long-term IL-1β direct osteogenic differentiation hMSCs vitro. IL-1β-stimulated cells showed enhanced proliferation entered maturation prior to non-stimulated ones, as monitored by ALP activity. The calcification was accelerated during stimulation with IL-1β. Since donor variability a well-known issue, we suggest new method illustrate global changes random chosen population through collative analysis. further demonstrate an absorbance assay evaluate degree vitro culture monolayer expanded hMSCs. Our findings support importance model. A online useful at early stages. These will be helpful optimizing predifferentiation for tissue engineering. Copyright © 2014 John Wiley & Sons, Ltd.
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