Binding stoichiometry and structure of RecA-DNA complexes studied by flow linear dichroism and fluorescence spectroscopy: Evidence for multiple heterogeneous DNA Co-ordination
作者： Masayuki Takahashi , Mikael Kubista , Bengt Nordén
DOI: 10.1016/0022-2836(89)90371-9
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摘要: The interaction between RecA and DNA (in the form of unmodified single-stranded DNA, fluorescent double-stranded DNA) is studied with linear dichroism fluorescence spectroscopy. found to a complex binding stoichiometry about four nucleotides per monomer, in which bases appear have random orientation. Addition ATP gamma S (a non-hydrolyzable analog ATP) reduces three causes adopt an orientation preferentially perpendicular fiber axis. This can incorporate additional strand or yielding total six base-pairs, respectively, RecA. RecA, presence S, also interact base-pairs In all complexes, tryptophan residues protein are oriented their planes parallel axis, whereas complexes involving fiber. virtually excludes possibility that intercalated helix. On basis these results, model for research homology RecA-mediated, strand-exchange reaction genetic recombination process proposed.
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