Effects of Energy Metabolism on in Vivo Distribution of Hexokinase in Brain

摘要: Abstract Hexokinase partitioning between the soluble and mitochondrial fractions of brain has been observed to vary according energy status brain. Ischemia, 0.5 2.5 min, in chick led an increased hexokinase activity fraction cerebellar homogenates from 4.4 ± 0.84 7.6 0.66 µmoles per min g fresh weight; correspondingly, decreased 3.9 0.60 1.6 0.28 weight. Feeding galactose chicks, which is known decrease adenine nucleotide charge levels several glycolytic intermediates, resulted a redistribution both cerebellum cerebrum, mimicking changes that occur during ischemia. Intraperitoneal injection glucose into chicks fed caused increases ATP 6-phosphate those control animals. Concomitantly, activities readjusted toward values chicks. A regional difference was distribution The percentage 47% as compared with 53% form, whereas corresponding were 31 69%. These distributions correlate differences content. Cerebellar 2.51 0.39 g, while cerebral 1.91 0.17. In vitro studies indicated solubilization most sensitive at these concentrations ATP. observations indicate vivo ⇄ equilibrium responsive posture are consistent involvement such regulation carbohydrate metabolism.