Novel retinoid-binding proteins from filarial parasites.

摘要: The present study deals with the discovery and partial characterization of specific binding proteins for retinol retinoic acid from filarial parasites (worms superfamily Filarioidea), including those two species Onchocerca. These proteins, which are distinct in their physicochemical properties mode ligand interactions host-tissue retinoid-binding may be involved mediation putative biological roles retinoids control parasitic growth, differentiation reproduction. Parasite retinol-binding protein acid-binding exhibited specificity respectively. Both showed an s20,w value 2.0 S. On gel filtration, both were retarded to a position corresponding same molecular size (19.0 kDa). preparative columns, parasite isoelectric points at pH 5.7 5.75. Unlike mammalian avian origin, lack mercurial sensitivity binding. comparative amounts five parasites, Onchocerca volvulus, gibsoni, Dipetalonema viteae, Brugia pahangi Dirofilaria immitis, between 2.7 3.1 pmol bound/mg extractable protein. However, levels 4.8 5.8 pmol/mg, is considerably higher than cellular origin. retinol- acid-binding-protein O. volvulus-infected human nodules gibsoni-infected bovine similar tissues. Also, these nodular like host-binding interactions.