Platelet Proteomics: State of the Art and Future Perspective

摘要: Platelets pose unique challenges to cell biologists due their lack of nucleus and low levels messenger RNA. cannot be cultured in great abundance or manipulated using common recombinant DNA technologies. As a result, platelet research has lagged behind that nucleated cells. The advent mass spectrometry its application protein biochemistry brought with it hopes for the community are now being realized. This technology is ideally suited identifying low-abundance proteins, protein-protein interactions, post-translational modifications complex mixtures. Over past 10 years, proteomics delivered many ways, providing comprehensive list proteins expressed platelets, information on modifications, interactions sub-cellular localization. Several novel important membrane including CLEC-2, CD148, G6b-B, G6f, Hsp47, have been identified proteomics-based approaches. New, more sensitive instrumentation approaches making increasingly possible identify ever lower amounts proteins. In this chapter we highlight some major achievements date, discussing how they were overcome. We also discuss new frontiers applications platelets microparticles health disease, as strive better understand molecular mechanisms underlying response vascular injury.