作者: Ichiro Hara , Mitsuyo Okazaki
DOI: 10.1016/0076-6879(86)29062-X
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摘要: Publisher Summary High-performance liquid chromatography (HPLC) has been widely used for the separation and analysis of many substances due to short experimental time, small sample analysis, high resolution, excellent reproducibility. A simple rapid method lipoprotein developed using HPLC with gel permeation column. direct quantitation cholesterol in each class a very amount intact serum through combination methods (1) by column, (2) selective detection post column effluent an enzymatic reaction reagent. The technique monitoring lipids holds promise as means from following standpoints: simplicity procedure, time less than one hour, no need pretreatment, required (less 20/μl serum), good