Size control of in vitro synthesized magnetite crystals by the MamC protein of Magnetococcus marinus strain MC-1.

摘要: Magnetotactic bacteria are a diverse group of prokaryotes that share the unique ability biomineralizing magnetosomes, which intracellular, membrane-bounded crystals either magnetite (Fe3O4) or greigite (Fe3S4). Magnetosome biomineralization is mediated by number specific proteins, many localized in magnetosome membrane, and thus under strict genetic control. Several studies have partially elucidated effects these magnetosome-associated proteins control size crystals. However, effect MamC, one most abundant remains unclear. In this present study, nanoparticles were synthesized inorganically free-drift experiments at 25 °C presence different concentrations iron-binding recombinant MamC MamCnts (MamC without its first transmembrane segment) from marine, magnetotactic bacterium Magnetococcus marinus strain MC-1 three commercial [α-lactalbumin (α-Lac), myoglobin (Myo), lysozyme (Lyz)]. While no was observed on formed biomimetic synthesis 10–60 μg/mL resulted production larger more well-developed (~30–40 nm) compared to those (~20–30 nm; grown protein-free). Our results demonstrate plays an important role could be utilized nanocrystals.