Detection and identification of Kinetoplastids of zoonotic interest by HRM-qPCR analysis in Canis lupus familiaris from Argentinean Mesopotamia.

摘要: Abstract This work aimed to conduct a first PCR-based approach for differential diagnosis of kinetoplastidean infections in dogs. Diagnosis Kinetoplastid domestic animals is difficult, since parasitemia intermittent and signs are nonspecific; it mainly based on parasitological smears or concentration techniques, which lack sensitivity depend operator` expertise. Dogs relevant reservoirs transmission Kinetoplastids; they function as sentinels detect active cycles before involve humans. Trypanosoma cruzi, evansi, various species Leishmania genus multi-host parasites, capable parasitizing dogs among vast number reservoirs. An algorithm sequential Real-Time PCR-High Resolution Melting (HRM) (qPCR-HRM) assays directed at 24S alpha ribosomal DNA, ITS1 Hsp70 designed distinguish T. rangeli, evansi spp. was tested fourteen with suspicion kinetoplastid diseases. A qPCR control DNA integrity the sample, targeted mammalian interphotoreceptor retinoid-binding protein (IRBP) gene fragment incorporated algorithm. detected four L. infantum one. Two five positive cases were smear negative. Smear corresponded that died despite being treated, indicating association disease severity. laboratory tool increases possibility confirming outbreaks diseases zoonotic potential identify etiological agents involved.