Co-expression of laminin β3 and γ2 chains and epigenetic inactivation of laminin α3 chain in gastric cancer.

摘要: Laminin-332 (LM-332, formerly termed laminin-5) is a heterotrimeric glycoprotein that regulates cell adhesion and migration. Molecular alterations of LM-332 are involved in cancer progression. The aim this study was to clarify gastric carcinoma. expression subunits 10 carcinoma lines investigated by RT-PCR, Western blotting, immuno-cytochemical/immunofluorescent analyses. promoter methylation status LM-332-encoding genes (LAMA3, LAMB3 LAMC2) analyzed methylation-specific PCR (MSP). relationship between migration assessed the scratch assay. immunohistochemically 90 tissues. Co-expression laminin β3 γ2 chains often observed at mRNA protein levels. In contrast, there no α3 either or Extra-cellular secretion found 2 lines. LAMA3 gene transcriptionally silenced CpG islands all lines, while LAMC2 were several Treatment with demethylating agent, 5-aza-2'-deoxycytidine (5-aza-dC), restored genes. Methylation frequency higher than those LAMB2 Migration distances significantly correlated cytoplasmic chain expression. Immunohistochemistry showed frequent co-expression cells, which depth invasion advanced tumor stage. results suggest accumulate intracellularly play role progression, epigenetic silencing may lead inability synthesize basement membrane affect invasion. Cancer motility appears be associated cyto-plasmic vitro.