Chapter 4.4. Adsorption and partition chromatography

摘要: Publisher Summary This chapter discusses adsorption and partition chromatography. Adsorption chromatography is based on liquid–solid partition—that is, a of solute molecules between the liquid mobile phase active sites surface solid stationary phase. The support used most often silica or alumina, former being far more widely used. During chromatographic process are adsorbed to in competition with solvent molecules. consist hydroxyl groups (for silanol groups, Si-OH) affinity them greater polar molecules, their ability form hydrogen bondings. structures different brands largely identical, thus selectivity towards various mixtures solutes does not exhibit great variation. Separation by liquid–liquid result difference distribution two immiscible phases individual components mixture. coated support, which ideally inert. As relatively large pore (10–50 nm diameter) Coating onto may be performed ways. evaporation technique (as preparation GC column materials) used, provided that non-volatile particle size exceeds 20 μm.