Alternative Pathways of Glucose Metabolism III. The Incorporation of Radioactivity from Glucose-1-C14 into the Nucleic Acids of Regenerating Rat Liver

摘要: Summary 1.The rate of excretion radioactivity following the intraperitoneal injection 10 µc. a -d-glucose-1-C 14 into rats with 24-hour regenerating livers was examined. It found that 83 per cent dose excreted as carbon dioxide by 12 hours after and an additional 4.5 in urine. These results were higher than values previously obtained tumor-bearing rats. 2.Rats injected at different times partial hepatectomy sacrificed 2 injection. Incorporation during this 2-hour period used measure degree stimulation synthesis given type nucleic acid. 3.Based on criterion, it for nuclear cytoplasmic ribonucleic acid (RNA) increased almost immediately operation. In contrast, there no deoxyribonucleic (DNA) until 18 At time, DNA could be detected, rose to maximum 30 4.When 24 operation injection, specific activity (S.A.) RNA reached After S.A. not significantly different, both types decreased slowly postinjection, so 4 weeks only small amount remained these fractions. The 96 By still high level. decrease observed attributed dilution rather destruction radioactive DNA. 5.The rapidly glucose then exponentially between 6 possible significance exponential disappearance from nucleus discussed.