Bacteriophage therapy: a novel method of lytic phage delivery

摘要: The widespread emergence of muki-antibiotic resistant bacteria has increased the need for alternatives to conventional antibiotic therapy. Accordingly, a significant amount effort been made investigate potential use bacteriophages as prophylactic and therapeutic agents bacterial infections. In this study, molecular biological techniques were applied construct iysogen lytic bacteriophage X in an attempt combat with multi-antibiotic by novel method phage delivery. To accomplish goal, two plasmid-based site-specific recombination (SSR) systems integration recovery DNA constructs from Escherichia coli Salnumella typhimurium chromosomes developed. are mediated SSR machineries X. £1 P22 S. typhimurium. These utilize plasmid vectors conditional replicating origin replication provide stable chromosomal genes at specific attachment sites without disruption any host gene or selection. E. contains both bacteriophages. When consecutively, different can be integrated locations. plasmids also completely excised recovered fi'om observe genetic changes, e.g. sequencing. Both very applicable construction strains well live recombinant vaccines expressing foreign interest. Iysogen X, applied. A mutant {ct) was marked cassette