Down-Regulation of ZEB1 by miR-199a-3p Overexpression Restrains Tumor Stem-Like Properties and Mitochondrial Function of Non-Small Cell Lung Cancer.

摘要: Objective MicroRNA-199a-3p (miR-199a-3p or miR-199b-3p) targeting of 3'-UTR ZEB1 was characterized as an important way to inhibit invasion and metastases in non-small cell lung cancer (NSCLC), one the most common cancers around world. Here we aimed investigate tumor-suppressive role miR-199a-3p targeted ZEB1. Materials methods A549 cells were transfected with and/or miR-199a-3p. Then, tumor growth investigated xenograft mice. Stem-like property, proliferation mitochondria injury further validated vitro. Results Overexpression premiRNAs significantly reduced inhibited CD44 Ki67 increased Caspase-3 Sphere formation protein expression stem-like markers showed that stemness cell. cells, EdU staining Ki67. Transfection also promoted apoptosis, indicated apoptotic flow cytometry, cleaved Caspase-3/Caspase3 Bcl-2/Bax. Apoptosis be induced dysfunction, which JC-1 labeled loss mitochondrial membrane potential, activity SOD, MDA LDH. All these effects inverted overexpression Conclusion Altogether, findings suggested up-regulation NSCLC vivo, mitochondrial-mediated through down-regulation The supported down-expression a novel therapeutic target for treatment.