HIF-Mediated Hypoxic Response is Missing in Severely Hypoxic Uterine Leiomyomas

摘要: Results from our laboratory have put a question mark on the existence of direct quantitative relationship between tumor hypoxia and HIF-mediated protein expression in cancers uterine cervix. In present study, this subject has been further explored by analysis HIF-related marker benign entity - leiomyomas using immunohistochemistry, western blotting RT-PCR. The oxygenation status 17 was assessed means intraoperative polarographic needle electrode measurements. show that these tumors are severely uniformly hypoxic, but do not induce HIF-1alpha, HIF-2alpha, glucose transporter (GLUT)-1 or carbonic anhydrase (CA) IX. Furthermore, downregulation HIF-system caused an overexpression hypoxia-inducible prolyl hydroxylase domain proteins (PHDs) 2 3. Compared with normal myometrium, also poorer vascularization. Conversely, leiomyosarcomas abundant markers despite similar microvascular density. conclusion, results indicate strong activation HIF system observed solid malignant may be mechanistically linked to their transformed phenotype, rather than being physiological reaction activated pathological context.