In silico and in vivo studies of Astragalus glycyphylloides saponin(s) with relevance to metabolic syndrome modulation

摘要: Abstract Triterpenoids are well known modulators of metabolic syndrome. One the suggested modes action (MoAs) involves peroxisome proliferator-activated receptor gamma (PPARγ) binding. In this study we aimed to: (i) evaluate in silico potential metabolites and PPARγ-mediated MoA sapogenin main saponin present a purified saponins' mixture (PSM) from Astragalus glycyphylloides; (ii) estimate vivo PSM's toxicity; (iii) investigate antihyperglycaemic, hypolipidaemic, antioxidant hepatoprotective effects PSM. Metabolites toxicity were predicted using Meteor Derek Nexus expert systems (Lhasa Limited) PPARγ binding was investigated software MOE (CCG Inc.). acute oral evaluated mice pharmacological assessed streptozotocin-induced diabetic spontaneously hypertensive rats (SHRs). Liver histopathology studied as well. weak partial agonism for 24 probable/plausible Phase I which docking poses clustered 12 different with characteristic protein-ligand interactions. beneficial on levels blood glucose, triglycerides, total cholesterol, oxidative stress markers liver histology SHRs comparable to those ligand pioglitazone. safety profile confirmed vivo.