Spatial distribution and catalytic mechanisms of β-glucosidase activity at the root-soil interface

摘要: We compared modifications of soil zymography, a new in situ technique to visualize enzyme activities, based on contact fluorgenic substrate-saturated membranes with either through the gel layer (gel zymography) or without application (direct zymography). coupled zymography quantitative measurements kinetics characterize catalytic mechanisms β-glucosidase activity at plant-soil interface including root surface (rhizoplane), rhizosphere, and bulk soil. Direct refined focused image resolution. The area hotspots (i.e., spots most intensive activity) as well color intensity ratios estimated using direct exceeded by factor 2 corresponding values obtained zymography. As determined percentage associated surfaces was 58–68 % total hotspot area. Hotspot comprised only 6.8 ± 0.1 an 9.0 3 activity, however, up 20 times higher versus contribution rhizosphere whole (77–82 %) four than surface. Enzyme kinetic parameters indicated different systems Higher substrate affinity efficiency suggested relative domination microorganisms more efficient former. Coupling assays enabled mapping two-dimensional (2D) distribution root-soil estimating properties root-associated soil-associated enzymes.