Molecular Detection of Theileria annulata in Cattle from Different Regions of Punjab, Pakistan, by Using Recombinase Polymerase Amplification and Polymerase Chain Reaction
作者： Muhammad Adeel Hassan , Junlong Liu , Muhammad Sohail Sajid , Altaf Mahmood , Shuaiyang Zhao
DOI: 10.1645/17-173
关键词:
摘要: Abstract Piroplasmosis is one of the most important diseases livestock, constraining optimal production and leading to economic loss. This study was carried out detect Theileria annulata by using 2 different molecular techniques: recombinase polymerase amplification (RPA) conventional chain reaction (PCR). Blood samples were collected from 274 ticks infesting asymptomatic cattle several counties in Chakwal, Faisalabad, Jhang districts Punjab Province Pakistan FTA cards. After extraction genomic DNA, each sample subjected RPA optimized amplify a 281-bp fragment Enolase gene for T. annulata. The specificity test confirmed positive DNA related piroplasm species, whereas analytical sensitivity calculated serial dilutions long same gene. results 48 (17.51%) samples. All screened PCR, 21 (7.66%) samp...
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