Measles and Rubella Viruses

摘要: This chapter combines the current laboratory diagnostic methods for measles virus and rubella convenient review reference. The genome is 15,894 nucleotides in length contains six structural genes organized on single strand of RNA a gene order consistent with those most paramyxoviruses, i.e., 3'-N, P. M, F, H, L-5'. A recent by Rota et al. provides an excellent overview status molecular epidemiology global distribution various genotypes. common complications associated infection are otitis media (7 to 9%), pneumonia (1 6%), diarrhea (6%). Suitable samples isolation or detection viral antigen can be whole blood, serum, throat nasopharyngeal secretions, urine, and, special circumstances, brain skin biopsy samples. Characteristic cytopathic effects (CPE) include multinucleated cells cellular inclusions (in-tracytoplasmic intranuclear). reverse transcriptase PCR (RT-PCR) should considered use where IgM testing compromised concurrent virus-containing vaccine as part outbreak response settings distribution, such supplemental immunization activities. Groups related viruses within clades have been classified Time course virus-specific IgG enzyme-linked immunosorbent assays (ELISAs) sera patients.