The Studies on the Aetiology of Diarrhoea in Neonatal Calves and Determination of Virulence Gene Markers of Escherichia coli Strains by Multiplex PCR
作者： M. Ok , L. Güler , K. Turgut , Ü. Ok , I. Şen
DOI: 10.1111/J.1863-2378.2008.01156.X
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摘要: Summary The purpose of this study was to determine aetiological agents diarrhoea in neonatal calves and investigate virulence gene markers Escherichia coli strains isolated from by multiplex polymerase chain reaction (PCR). Eighty-two diarrhoeic 18 healthy were used as subjects. Faeces taken the rectums all subjected bacterial culture. Antigen enzyme-linked immunosorbent assay (ELISA) performed detect rotavirus, coronavirus E. K99 faeces calves. A PCR characterize 37 samples, Enterococcus ssp. 22 samples Salmonella one sample Furthermore, only Of calves, (18.9%), F41 heat-stable enterotoxin a (STa) Shiga toxin 1 (Stx1; 13.5%) 2 (Stx2; 5.4%) intimin (8.1%) genes identified PCR. (16.6%) (55.5%) total 15 11 detected antigen ELISA. As result, shows that coronavirus, determined play role aetiology K99, F41, STa, Stx1 Stx2 found most common with diarrhoea. Multiplex may be useful for characterization
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