New alkaline protease from Nocardiopsis sp.: partial purification and characterization
作者： K.A. Moreira , T.S. Porto , M.F.S. Teixeira , A.L.F Porto , J.L. Lima Filho
DOI: 10.1016/S0032-9592(02)00312-6
关键词:
摘要: Abstract A new alkaline protease from Nocardiopsis sp., isolated a soil sample collected the Northeast of Brazil is reported. The crude extract (CE) was designate as partially purified (PPE) after purification with ammonium sulphate (0–20%). Optimal pH and temperature for detection activity were obtaining at 10.5 8.0, CE PPE, respectively, 50 °C both extracts. enzyme stable more than 75% retained even incubation 120 min pH's between 8.0 10.5, use specific inhibitors, made it possible to show that belongs group serine protease. Maximum achieved (50 U/mg) 10.0 PPE (1260 U/mg). partial Sephadex G-75 obtained 26-purification fold 34% yield.
elsevier.com
sci-hub.se 参考文章(30)
A. M. Wolff, M. S. Showell, M. G. Venegas, B. L. Barnett, W. C. Wertz, Laundry Performance Of Subtilisin Proteases Advances in Experimental Medicine and Biology. ,vol. 379, pp. 113- 120 ,(1996) , 10.1007/978-1-4613-0319-0_12
E. Gary Myers, Soap and Detergents Springer Netherlands. pp. 149- 176 ,(1992) , 10.1007/978-94-011-7933-1_7
Christian Betzel, Richard Bott, Subtilisin enzymes : practical protein engineering Plenum Press. ,(1996)
Claus Dambmann, Dorrit A. Aaslyng, Helle Outtrup, Margrethe Christiansen, Alkaline protease Bacillus sp. JP 395, method of making and detergent compositions ,(1992)
Padmavathi Sampath, Gowri Chandrakasan, Chitra Subramanian, Extracellular proteases from Streptomyces spp. G157: purification and characterization Biotechnology and Applied Biochemistry. ,vol. 26, pp. 85- 90 ,(1997) , 10.1111/J.1470-8744.1997.TB00451.X
M. Pokorny, Lj. Vitale, V. Turk, M. Renko, J. Žuvanić, Streptomyces rimosus extracellular proteases European Journal of Applied Microbiology and Biotechnology. ,vol. 8, pp. 81- 90 ,(1979) , 10.1007/BF00510269
D R Durham, D B Stewart, E J Stellwag, Novel alkaline- and heat-stable serine proteases from alkalophilic Bacillus sp. strain GX6638. Journal of Bacteriology. ,vol. 169, pp. 2762- 2768 ,(1987) , 10.1128/JB.169.6.2762-2768.1987
M J North, Comparative biochemistry of the proteinases of eucaryotic microorganisms. Microbiological Research. ,vol. 46, pp. 308- 340 ,(1982) , 10.1128/MR.46.3.308-340.1982
Sangita U. Phadatare, Vasanti V. Deshpande, Mandyam C. Srinivasan, High activity alkaline protease from Conidiobolus coronatus (NCL 86.8.20) : enzyme production and compatibility with commercial detergents Enzyme and Microbial Technology. ,vol. 15, pp. 72- 76 ,(1993) , 10.1016/0141-0229(93)90119-M
A. L. F. Porto, G. M. Campos-Takaki, J. L. Lima Filho, Effects of culture conditions on protease production by Streptomyces clavuligerus growing on soy bean flour medium Applied Biochemistry and Biotechnology. ,vol. 60, pp. 115- 122 ,(1996) , 10.1007/BF02788066