Lack of dependence of amine or prostaglandin biosynthesis on absolute cerebral level of pteridine cofactor

摘要: Abstract Using 2-amino-6-(5'-2'-deoxyphosphoribosyl)-amino-5- or -6-formamido-6-hydroxypyrimidine (dFPyd-P3), a specific inhibitor of tetrahydrobiopterin (BH4) synthesis, cerebral pools BH4 were reduced to half that controls; while, simultaneously, the biosynthesis de novo L-erythrodihydroniopterin (BH2) from GTP was inhibited by about 98%. Nevertheless, there no effect on levels serotonin, dopamine, norepinephrine prostaglandin E2 F1. The data are presented in evidence absolute level cofactor is not regulatory amine protaglandin vivo . Amine and proceeded even at concentrations 9×107 M nsuggesting their dependent rate H+ + e shuttle between BH2 BH4.