Structure of the Sec13/31 COPII coat cage
作者： Scott M. Stagg , Cemal Gürkan , Douglas M. Fowler , Paul LaPointe , Ted R. Foss
DOI: 10.1038/NATURE04339
关键词:
摘要: Endomembranes of eukaryotic cells are dynamic structures that in continuous communication through the activity specialized cellular machineries, such as coat protein complex II (COPII), which mediates cargo export from endoplasmic reticulum (ER). COPII consists Sar1 GTPase, Sec23 and Sec24 (Sec23/24), where is a Sar1-specific GTPase-activating functions selection, Sec13 Sec31 (Sec13/31), has structural role. Whereas recent results have shown Sec23/24 Sec13/31 can self-assemble to form cage-like particles, we now show minimal cages absence Sec23/24. We present three-dimensional reconstruction these at 30 A resolution using cryo-electron microscopy single particle analysis. These reveal novel cuboctahedron geometry with potential flexible lattice generate diverse range containers. Our data consistent model for assembly non-structural role multivalent ligand localizing self-assembly cage driving ER export.
nature.com
unirioja.es
harvard.edu
sci-hub.se 参考文章(29)
N.R. Salama, T. Yeung, R.W. Schekman, The Sec13p complex and reconstitution of vesicle budding from the ER with purified cytosolic proteins The EMBO Journal. ,vol. 12, pp. 4073- 4082 ,(1993) , 10.1002/J.1460-2075.1993.TB06091.X
Andrea Musacchio, Corinne J Smith, Alan M Roseman, Stephen C Harrison, Tomas Kirchhausen, Barbara M.F Pearse, Functional Organization of Clathrin in Coats: Combining Electron Cryomicroscopy and X-Ray Crystallography Molecular Cell. ,vol. 3, pp. 761- 770 ,(1999) , 10.1016/S1097-2765(01)80008-3
Yuval Shimoni, Randy Schekman, Vesicle budding from endoplasmic reticulum. Methods in Enzymology. ,vol. 351, pp. 258- 278 ,(2002) , 10.1016/S0076-6879(02)51852-8
William J. Belden, Charles Barlowe, Purification of functional Sec13p-Sec31p complex, a subunit of COPII coat. Methods in Enzymology. ,vol. 329, pp. 438- 443 ,(2001) , 10.1016/S0076-6879(01)29105-8
F. Gorelick, C.A. Shugrue, E.R. Kolen, L. Matovcik, C. Kaiser, H. Peters, A. Czernik, A.L. Hubbard, Identification of the putative mammalian orthologue of Sec31P, a component of the COPII coat. Journal of Cell Science. ,vol. 112, pp. 4547- 4556 ,(1999) , 10.1242/JCS.112.24.4547
Juan S Bonifacino, Benjamin S Glick, The Mechanisms of Vesicle Budding and Fusion Cell. ,vol. 116, pp. 153- 166 ,(2004) , 10.1016/S0092-8674(03)01079-1
G. Z. Lederkremer, Y. Cheng, B. M. Petre, E. Vogan, S. Springer, R. Schekman, T. Walz, T. Kirchhausen, Structure of the Sec23p/24p and Sec13p/31p complexes of COPII. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 10704- 10709 ,(2001) , 10.1073/PNAS.191359398
Bethan Jones, Emma L Jones, Stephanie A Bonney, Hetal N Patel, Arjen R Mensenkamp, Sophie Eichenbaum-Voline, Mats Rudling, Urban Myrdal, Grazia Annesi, Sandhia Naik, Nigel Meadows, Aldo Quattrone, Suhail A Islam, Rossitza P Naoumova, Bo Angelin, None, Mutations in a Sar1 GTPase of COPII vesicles are associated with lipid absorption disorders Nature Genetics. ,vol. 34, pp. 29- 31 ,(2003) , 10.1038/NG1145
Jinoh Kim, Susan Hamamoto, Mariella Ravazzola, Lelio Orci, Randy Schekman, Uncoupled Packaging of Amyloid Precursor Protein and Presenilin 1 into Coat Protein Complex II Vesicles Journal of Biological Chemistry. ,vol. 280, pp. 7758- 7768 ,(2005) , 10.1074/JBC.M411091200
Ken Matsuoka, Lelio Orci, Mylène Amherdt, Sebastian Y Bednarek, Susan Hamamoto, Randy Schekman, Thomas Yeung, COPII-Coated Vesicle Formation Reconstituted with Purified Coat Proteins and Chemically Defined Liposomes Cell. ,vol. 93, pp. 263- 275 ,(1998) , 10.1016/S0092-8674(00)81577-9