Protein Phosphatase 1 and 2A Inhibitors Prolong the Switch in the Control of Glutamate Release by Group I Metabotropic Glutamate Receptors

摘要: We have addressed the role of protein phosphatases (PPs) in modulation switch glutamate release observed after repetitive stimulation group I metabotropic receptors (mGluRs). In cerebrocortical nerve terminals agonist (S:)-3, 5-dihydroxyphenylglycine facilitated evoked release. However, a second stimulation, 5 min later, reduced rather than this This control was reversed when 30-min interval left between stimulations. Inhibition endogenous PPs, PP1 and PP2A, with calyculin A okadaic acid prevented recovery facilitatory response maintained receptor permanently coupled to inhibitory pathway. The inhibitors PP2B, cyclosporin cypermethrine, had no effect. inhibition insensitive pertussis toxin result loss component N-type Ca(2+) channels. action suppressed by addition kinase C activator 4beta-phorbol 12,13-dibutyrate. conclude that balance phosphatase activity at terminal plays key accommodating mGluRs.