Identification of major slowly growing pathogenic mycobacteria and Mycobacterium gordonae by high-performance liquid chromatography of their mycolic acids.

摘要: A rapid, reverse-phase high-performance liquid chromatography method was used to detect rho-bromophenacyl mycolic acid ester patterns for strains of four major pathogenic Mycobacterium species and the most commonly encountered saprophytic species, gordonae. Mycobacteria in low numbers (2.5 X 10(6) CFU) were detected identified level. Standard chromatographic characteristic each established. Simple pattern recognition enabled rapid identification M. tuberculosis, kansasii, avium, intracellulare,