Flavanol Quantification of Grapes via Multiple Reaction Monitoring Mass Spectrometry. Application to Differentiation among Clones of Vitis vinifera L. cv. Rufete Grapes.

作者： Ignacio García-Estévez , Cristina Alcalde-Eon , M. Teresa Escribano-Bailón

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摘要: The determination of the detailed flavanol composition in food matrices is not a simple task because structural similarities monomers and, consequently, oligomers and polymers. aim this study was development validation an HPLC-MS/MS–multiple reaction monitoring (MRM) method that would allow accurate precise quantification catechins, gallocatechins, oligomeric proanthocyanidins. high correlation coefficients calibration curves (>0.993), recoveries statistically different from 100%, good intra- interday precisions (<5%), LOD LOQ values, low enough to quantify flavanols grapes, are results procedure. Its usefulness has also been tested by determining Vitis vinifera L. cv. Rufete grapes. Seventy-two (38 nongalloylated 34 galloylated) 53 (24 procyanidins 29 prodelphinidins) have identified quantified grape seed skin, respect...

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Flavanol Quantification of Grapes via Multiple Reaction Monitoring Mass Spectrometry. Application to Differentiation among Clones of Vitis vinifera L. cv. Rufete Grapes.

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Flavanol Quantification of Grapes via Multiple Reaction Monitoring Mass Spectrometry. Application to Differentiation among Clones of Vitis vinifera L. cv. Rufete Grapes.

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