Proteolytic processing of polyproteins 1a and 1ab between non-structural proteins 10 and 11/12 of Coronavirus infectious bronchitis virus is dispensable for viral replication in cultured cells
作者： Shou Guo Fang , Hongyuan Shen , Jibin Wang , Felicia P.L. Tay , Ding Xiang Liu
DOI: 10.1016/J.VIROL.2008.06.038
关键词:
摘要: Coronavirus 3C-like proteinase (3CLpro) plays important roles in viral life cycle through extensive processing of the polyproteins 1a and 1ab into 12 mature, non-structural proteins (nsp5-nsp16). Structural biochemical studies have revealed that all confirmed 3CLpro cleavage sites a conserved Gln residue at P1 position, which is thought to be absolutely required for efficient cleavage. Recent on murine hepatitis virus (MHV) showed polyprotein position between nsp10-nsp11 essential replication. In this report, we investigated requirement equivalent replication avian coronavirus infectious bronchitis (IBV), using an cloning system. The results mutation Pro or deletion nsp10-nsp11/12 site completely abolished 3CLpro-mediated processing, but allowed production recombinant viruses with variable degrees growth defect, suggesting IBV dispensable cultured cells. This study would pave way potential vaccine development by generation attenuated from field isolates manipulation site. Similar approaches also applicable other human animal coronaviruses.
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