Amidases of Pseudomonas aeruginosa

摘要: In choosing an enzyme of Pseudomonas aeruginosa as the starting point for studies in experimental evolution, we had mind well-known biochemical versatility species. Many catabolic pathways been described detail species belonging to this genus, and biochemists interested isolating strains that could attack unusual organic compounds often found isolates obtained from enrichment cultures be assigned group. A very comprehensive survey laboratory carried out 60 years ago by Den Dooren De Jong (1926) pointed putida a particularly versatile organism. He one his utilize about 80 200 test sole carbon source growth. Among used Jong’s experiments were sodium salts bromosuccinic α- β-bromopropionic acids. These chemicals, it asked whether they attacked enzymes with wide substrate specificities not distinguish between halogenated nonhalogenated substrates or there specific evolved act on occur rarely if at all nature. Such questions become pertinent when is realized during last century activities chemical industry have released many synthetic chemicals into natural environment.