The hepatoprotective effect of sitagliptin against hepatic ischemia reperfusion-induced injury in rats involves Nrf-2/HO-1 pathway.
作者： Seham A. Abdel-Gaber , Ayman Geddawy , Rabab A. Moussa
DOI: 10.1016/J.PHAREP.2019.06.006
关键词:
摘要: Abstract Background Oxidative stress and inflammation play a key role in the development of hepatic ischemia reperfusion (HIR)-induced injury. Nuclear factor-erythroid 2-related factor-2 (Nrf-2) is main regulator numerous genes, encoding cytoprotective molecules including heme oxygenase-1 (HO-1). Sitagliptin (Sit) an incretin enhancer acting via inhibition dipeptidyl peptidase-4 (DPP-4) enzyme. This study was undertaken to investigate ability Sit prevent pathological changes HIR induced injury modify Nrf-2 its target HO-1. Methods Pringle's maneuver used induce total adult male rats that were randomly assigned into 4 groups. Group1 (sham-operated control), Group 2 (sham-operated + Sit-control group), 3 (HIR non-treated), (HIR + Sit). Alanine aminotransferase (ALT) aspartate (AST) activities together with contents malondialdhyde (MDA), nitric oxide (NO) reduced glutathione (GSH) superoxide dismutase (SOD) activity evaluated. Hepatic tissue mRNA protein content HO-1 along histopathological examination scoring performed. Results caused significant reduction ALT AST attenuation HIR-induced liver Effect associated decreased level MDA NO increased GSH SOD activity. Non-treated showed increase expression which further by treatment. Conclusions These results indicate hepatoprotective against attributed at least part modulation Nrf-2/ signaling pathway.
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