Nitroblue tetrazolium (NBT) reduction by bacteria. Some properties of the reaction and its possible use.

摘要: All the S. albus, E. coli and P. aeruginosa strains examined reduced nitroblue tetrazolium (NBT) to dark blue formazan. The amount of formazan produced was proportional number bacteria. Under same growth conditions, an equal bacteria various different amounts However, there were statistically verified differences in NBT-reduction between three species examined. took place all phases but most intense early logarithmic phase. NBT found be toxic for bacteria, had varying sensitivity that effect. NBT-reaction markedly enhanced by phenazine methosulphate (PMS). colour from has advantage over red triphenyltetrazolium chloride (TTC) if reaction occurs presence haemoglobin often present biological materials. With PMS, 10(6)--10(7) are needed form detectable may useful measuring influence bactericidal, bacteriostatic or growth-stimulating factors on