Recognition and signal transduction in the expression of Phytophthora resistance in soybean [Glycine max (L) Merr]

摘要: Phytophthora sojae, a hemibiotrophic oomycete, is the causal pathogen of stem and root rot disease soybean (Glycine max [L.] Merr). The annual yield loss suffered from this valued at about 300 million dollars. A series single Rps genes protect disease. In last two decades Rps1-k has been most stable widely used resistance gene in major soybean-producing regions United States. Two classes CC-NB-LRR-type were isolated locus. Representative members each class expressed transgenic plants. Analyses independent Ro, R1, RR2 R3 progeny populations suggested that both confer soybean. Sequence entire locus indicates it composed mostly repetitive sequences, including simple repeat tandem repeats retroelements. Gene content Rsp1-k region appears to be very poor, with only few full-length genes. yeast two-hybrid system was applied identifying signal transduction factors involved expression resistance. Various baits derived Rps1-k-2 screens. Thirteen putative Rps1-k-2-interacting proteins (RIP) identified based on vivo interactions vitro pull down assays. cells determined by FRET analysis preliminary RNA interference (RNAi) experiments transformed cotyledons further confirmed several candidate Rps1-k-2-interactors 26S proteasome AAA-ATPase subunit RPT5a, receptor kinase, an unknown protein type II metacaspase are promising Rps1-k-2-interactors. We characterized RIP13, showed