Green tea extract protects endothelial progenitor cells from oxidative insult through reduction of intracellular reactive oxygen species activity.

作者: Dian Nugrahenny , Indra Bachtiar , Dian Ratih Laksmitawati , Hana Ratnawati , Winsa Husin

DOI: 10.22038/IJBMS.2014.3334

关键词:

摘要: Objective(s): Many studies have reported that tea consumption decreases cardiovascular risk, but the mechanisms remain unclear. Green is known to potent antioxidant and free radical scavenging activities. This study aimed investigate whether green extract (GTE) can protect endothelial progenitors cells (EPCs) against oxidative stress through mechanisms. Materials Methods: Mononuclear (MNCs) were isolated from peripheral blood by density gradient centrifugation with Ficoll. The then plated on fibronectin-coated culture dishes. After 7 days of culture, EPCs characterized as adherent double positive for DiI-ac-LDL uptake lectin binding. further identified assessing expression CD34/45, CD133, KDR. treated hydrogen peroxide (H2O2) at doses 50, 100, 200 μM incubated or without GTE (25 μg/ml). intracellular reactive oxygen species (ROS) levels detected flow cytometry using a 2’,7’-dichlorofluorescein diacetate (DCF-DA) fluorescent probe. Results: ameliorated cell viability induced H2O2 about 25.47, 22.52, 11.96% higher than controls, respectively. also decreased ROS 84.24, 92.27, 93.72% compared respectively. Conclusion: improves reducing accumulation in H2O2-induced EPCs.

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