Dissection of glutathione conjugate turnover in yeast.
作者： Jana Wünschmann , Matthias Krajewski , Thomas Letzel , Eva M. Huber , Alexander Ehrmann
DOI: 10.1016/J.PHYTOCHEM.2009.09.034
关键词:
摘要: Xenobiotics are widely used as pesticides. The detoxification of xenobiotics frequently involves conjugation to glutathione prior compartmentalization and catabolism. In plants, degradation glutathione-S-conjugates is initiated either by aminoterminal or carboxyterminal amino acid cleavage catalyzed a γ-glutamyl transpeptidase phytochelatin synthase, respectively. order establish yeast model system for the analysis plant pathway, we monochlorobimane xenobiotic in Saccharomyces cerevisiae mutants thereof. catabolism form glutathione-S-bimane, which then turned over into γ-GluCys-bimane conjugate vacuolar serine carboxypeptidases CPC CPY. Alternatively, glutathione-S-bimane catabolized action Cis2p CysGly-conjugate. turnover was impaired cells deficient completely abolished additional inactivation CPY corresponding triple knockout. Inducible expression Arabidopsis synthase AtPCS1 knockout resulted observed plants. Challenge AtPCS1-expressing with zinc, cadmium, copper ions, known activate AtPCS1, enhanced accumulation. Thus, initial similar plants yeast, suitable study enzymes pathway.
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