作者: Björn Hendel , Jürgen Marxsen , Douglas Fiebig , Gudrun Preuß
DOI: 10.1016/S0043-1354(00)00538-8
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摘要: Abstract Activities of the extracellular enzymes β -glucosidase and phosphatase bacterial densities were investigated during filtration process at several sites in a groundwater recharge plant Ruhr river (Hengsen Schwerte, Germany). Low numbers microorganisms low levels activity this type habitat, compared to most surface waters, caused methodological problems when determining microbial activity. In study, fluorigenic model substrates, which enable hydrolytic rates as 1 nmol ( L × h ) −1 be measured, used determine enzyme activities. Highest activities determined water (107 nmol for 252 nmol phosphatase), decreased gravel prefilter main sand filter until end subsurface flow (1.6 nmol 6.8 nmol , respectively). Similarly, from 3.4×10 6 0.29×10 cells mL . These data showed that within shallow layers had greatest impact on quality. addition its involvement continuous purification water, community probably acts biological buffer against ephemeral increases loads organic matter nutrients plant.